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KMID : 1145120200180010041
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Asian Journal of Beauty and Cosmetology
2020 Volume.18 No. 1 p.41 ~ p.51
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Potential Anti-wrinkle Activity of Chlorella sp. HS1-derived Oil Components on Human Dermal Fibroblasts
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Kim Hye-Bin
Kim Ji-Yea
An Sung-Kwan
Lee Yong-Jae
Cho Dae-Hyun
Kim Hee-Sik
Bae Seung-Hee
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Abstract
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Purpose: In this study, we investigated the effects of Chlorella sp. HS1-derived oils on collagen expressions in dermal fibroblasts.
Methods: We prepared the oil extracts from Chlorella sp . HS1 biomass, and high-performance liquid chromatography analysis was used in the identification and quantitation of components in the oil extracts. Using human dermal fibroblasts, cell viability and quantitative RT-PCR (qRT-PCR)-mediated expression analysis of collagen and matrix metalloprotease (MMP) genes after oil treatments. We further investigated whether the effect of the oil extracts on collagen expression was related to the major component, β-carotene, of the extracts. In addition, we analyzed the effect of photoprotective effect of β-carotene on ultraviolet (UV)-irradiated dermal fibroblasts.
Results: We found that under 50 μg/mL of the oil extracts had little cytotoxicity in skin cells including epidermal keratinocytes, melanocytes and dermal fibroblasts. The HPLC analysis revealed that β-carotene was identified as the major non-polar molecules. Next, we found that the oil-derived β-carotene was little cytotoxicity in dermal fibroblasts. Further experiments revealed that treatment with the oil extracts significantly upregulated collagen type 1 and 3 mRNAs and downregulated MMP1 and MMP3 mRNAs in dermal fibroblasts. Also, we found that these effects were showed in β-carotene-treated cells. In addition, β-carotene significantly attenuated UVA-induced downregulation of collagen type 1 and 3 expressions.
Conclusion: Therefore, our results suggested that the oil extracts had a potential anti-wrinkle agent via its major component, β-carotene.
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KEYWORD
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Chlorella sp., Dermal fibroblasts, Anti-aging, Collagen, β-carotene
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